| Autor: |
Jiang, Yateng, Wang, Yanping, Luo, Wen, Luan, Xiaowei, Zhang, Zhibin, Pan, Yongchun, He, Bangshun, Gao, Yanfeng, Song, Yujun |
| Předmět: |
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| Zdroj: |
Lab on a Chip; 1/7/2025, Vol. 25 Issue 1, p49-56, 8p |
| Abstrakt: |
The intimate association between telomerase activity and cancer has driven the exploration of diverse methodologies for its precise detection. However, detecting telomerase activity at the single-cell level remains a significant challenge. Herein, we present a MOF–DNA barcode-amplified CRISPR-Cas12a strategy integrated with a single-cell microfluidic chip for ultrasensitive detection of telomerase activity. DNA-functionalized UiO-66 nanoparticles act as signal transducers, effectively converting telomerase activity into DNA activation strands, which subsequently trigger the trans-cleavage activity of CRISPR-Cas12a. This amplification-based assay could be integrated with a microfluidic chip to enable highly sensitive detection of telomerase activity at the single-cell level, offering promising advancements in early cancer diagnosis. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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