| Abstrakt: |
The study aimed to analyze the genetic background and functional gene characteristics of 27 strains of Lactobacillus amylovorus isolated from the intestines of Guanzhong black pigs at the genomic level. Whole-genome sequencing and draft genome assembly of the 27 strains of Lactobacillus amylovorus were completed using Illumina sequencing technology. Comparative genomics methods such as average nucleotide identity analysis, phylogenetic analysis, and pan-genome analysis were employed to analyze the homology and molecular evolutionary patterns among the strains. Functional gene annotation was conducted using databases such as eggNOG, CAZy, ResFinder, and VFDB. The results showed that the average genome sequence size of the 27 strains of Lactobacillus amylovorus was 1 881 796 bp, with an average GC content of 38.10%. The strains exhibited high homology and genetic diversity. Functional gene annotation revealed that these strains possess good survival capabilities and safety, as well as potential probiotic genes related to environmental stress, oxidative stress, riboflavin synthesis, extracellular polysaccharide secretion, and quorum sensing. The study indicates that the 27 strains of Lactobacillus amylovorus isolated from the intestines of Guanzhong black pigs are suitable as candidate probiotic strains. [ABSTRACT FROM AUTHOR] |
