| Autor: |
Facchin, Alessia, Ratti, Gabriele, Filipe, Joel, Penati, Martina, Gazzonis, Alessia L., Masiero, Greta, Dall'Ara, Paola, Alborali, Giovanni L., Lauzi, Stefania |
| Zdroj: |
Animals (2076-2615); Dec2024, Vol. 14 Issue 23, p3359, 13p |
| Abstrakt: |
Simple Summary: Antimicrobial resistance is a global health threat because microorganisms causing infections no longer respond to antimicrobial medicines, and this is particularly important in the case of resistance to last-resort antibiotics such as carbapenems. The role of dogs in the rise of antimicrobial resistance is under investigation. This study investigated the presence of Escherichia coli (E. coli) showing specific antimicrobial resistance patterns—defined as extended-spectrum β-lactamase (ESBL), AmpC and carbapenemase (CP) production—in dogs' feces from Northern Italy. Out of 100 samples, 14 (14%) dogs showed the presence of these resistant E. coli, with one (1%) bacterium showing resistance to carbapenems. Different combinations of resistance patterns were observed in these 14 E. coli. The majority (13/14, 92.9%) of the E. coli were also resistant to three or more different classes of antibiotics and were classified as multidrug-resistant. The presence of the resistant bacteria in feces tended to be associated with antibiotic treatment. The identification of bacteria known as ESBL-/AmpC-producing E. coli in domestic dogs, along with the presence of a bacterium resistant to carbapenems, although still limited, emphasizes the need for the adequate control of antimicrobial therapy and administration of antibiotics and surveillance programs for carbapenem-resistant bacteria in companion animals. MDR bacteria are an emerging global threat to public health, and the role of dogs in the rise of antimicrobial resistance is under investigation. This study investigated the fecal shedding of extended-spectrum β-lactamase (ESBL)-, AmpC- and carbapenemase (CP)-producing Escherichia coli and associated risk factors in dogs admitted to the Veterinary Teaching Hospital of Lodi, University of Milan, or other veterinary clinics and kennels in Northen Italy. Feces collected in 2020–2022 were microbiologically and molecularly analyzed. ESBL-/AmpC-/CP-producing E. coli was detected in 14/100 (14%) dogs. Eleven (11%), five (5%) and one (1%) dogs carried ESBL-, AmpC- and CP-producing E. coli phenotypes, respectively, supported by the PCR detection of blaCTX-M and/or blaTEM in ESBL-producing E. coli; blaCMY-2 and the presence of putative low-level AmpC production in AmpC-producing E. coli; and blaOXA-48 in CP-producing E. coli. Different combinations of resistance genes and genetic features were observed. Multidrug resistance was observed in 13/14 (92.9%) E. coli isolates. Binary logistic regression analysis showed that ESBL-/AmpC-/CP-producing E. coli fecal shedding tended to be associated with antibiotic treatment (p = 0.058; OR = 3.87). The detection of ESBL-/AmpC-producing E. coli, along with the presence of a carbapenemase-resistant E. coli isolate from domestic dogs, although still limited, emphasizes the need for antimicrobial stewardship and specific surveillance programs, particularly for CP-producing bacteria in companion animals. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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