| Autor: |
Grgurević, Karlo, Bramberger, Dora, Miloloža, Martina, Stublić, Krešimir, Ocelić Bulatović, Vesna, Ranilović, Jasmina, Ukić, Šime, Bolanča, Tomislav, Cvetnić, Matija, Markić, Marinko, Kučić Grgić, Dajana |
| Zdroj: |
Polymers (20734360); Dec2024, Vol. 16 Issue 23, p3407, 26p |
| Abstrakt: |
The environmental impact of plastic waste is a growing global challenge, primarily due to non-biodegradable plastics from fossil resources that accumulate in ecosystems. Biodegradable polymers like polyhydroxyalkanoates (PHAs) offer a sustainable alternative. PHAs are microbial biopolymers produced by microorganisms using renewable substrates, including agro-industrial byproducts, making them eco-friendly and cost-effective. This study focused on the isolation and characterization of PHA-producing microorganisms from agro-industrial waste, including chickpeas, chickpeas with bean residues, and starch. Screening via Sudan Black staining identified PHA-accumulating strains such as Brevibacillus sp., Micrococcus spp., and Candida krusei, among others. To assess the potential for PHA biosynthesis, solid-state fermentation (SSF) was conducted using agro-industrial waste as substrates, along with a mixed culture of the isolated microorganisms. The highest observed yield was a PHA accumulation of 13.81%, achieved with chickpeas containing bean residues. Structural and thermal characterization of the PHAs was performed using Fourier-transform infrared spectroscopy with attenuated total reflectance (FTIR-ATR), differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). FTIR-ATR spectra indicated polyhydroxybutyrate (PHB), suggesting it as the synthesized PHA type. This study highlights the potential of agro-industrial waste for sustainable PHA production and eco-friendly bioplastics. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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