| Abstrakt: |
Type-2 Diabetes Mellitus (T2DM), one of the most common metabolic illnesses, is characterized by elevated blood sugar levels caused by impaired insulin secretion, insulin action, or both. Plant-mediated green nanomaterial production is becoming increasingly popular due to its low cost and environmental friendliness. The study's goal is to manufacture Costus spicatus with AgNPs, characterize them by different analytical methods like UV-visible, FT-IR, SEM, TEM, XRD, and EDX spectroscopy, and conduct additional in vitro and in vivo experiments, different biomarkers SGOT, SGPT, and ALP were used;. In results UV‒visible analysis confirmed the successful bio-reduction of silver nanoparticles as Cs-AgNPs, its confirmed at 448 nm, indicated that SPR phenomenon, inFT-IR analysis peaks noticed at 619 cm⁻¹ and 453 cm⁻¹, it indicates C–Br and C–Cl bonding vibrations. In SEM analysis confirmed that the nanoparticles AgNPs were crystalline, spherical, and clustered, with sizes ranged 10 to 25 nm. In TEM analysis showed that homogeneous polycrystalline shapes 0.295 in size, XRD confirmed crystalline nature of AgNPs; at (311), (220), (200), and (111). In EDX the dominance of AgNPs observed (66.75%), followed by carbon (22.02%) and oxygen (11.23%). In antidiabbetic the animals treated with C. spicatus rhizome ethanol extract exhibited no damage in kidney its recovery observed at 0, 7, and 21 days. In 21 days observation of kidney body weight significantly increased and 21 days noticed that it returned to near-normal levels. Furthermore, in 0 days to 14 days observation the acute hyperlipidemia of TC, TG, LDL and HDL level of C. spicatus effects in STZ-induced levels decreased (SGOT: 108.39 ± 3.73# to 85 ± 1.92#, SGPT: 106 ± 2.19** to 73.67 ± 2.37#, ALP: 124.59 ± 2.37# to 80.17 ± 0.85**), indicating improved kidney function and anti-diabetic activity. These results, with **± 0.0001 indicating the significance of the control and silver nanoparticles as Cs-AgNPs STZ groups, treatment demonstrates significant kidney tissue restoration and promising anti-diabetic activity, offering potential in diabetes management strategies. silver nanoparticles as Cs-AgNPs together show promising in restoring kidney tissue and managing diabetes, its concluded that, the research has shown that the rhizome extract, when combined with nanoparticles, demonstrates potent antioxidant and enzyme inhibitory effects, especially targeting the key enzymes implicated in Type 2 Diabetes Mellitus (T2DM). [ABSTRACT FROM AUTHOR] |
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