| Abstrakt: |
The pathogenesis of type 1 and type 2 diabetes mellitus involve altering different immunological pathways which in turn affect host immune response and their genetic variations that will be affected as well. In order to overcome these physiological outcomes, many studies investigated the role of gene targeting to tackle diabetes. However, the role of immunoregulation and gene expression is still poorly studied. Therefore, this study aims to determine the role of signal regulatory protein alpha (SIRP-a) gene expression and related biochemical parameters in diabetes. 120 blood samples were collected (20 healthy and 100 diabetic patients), aged between (20-65) years, collected from Azadi Teaching Hospital in Kirkuk from February 2024 to April 2024. Samples were divided into three groups, the first group for patients with type 1 diabetes (Type 1) n= 50 samples, the second group for patients with type 2 diabetes (Type 2) n=50 samples, and the third group was 20 samples for healthy people. Results of the current study showed significant differences (P-Value < 0.01) between cumulative sugar, random blood sugar, fasting blood sugar, total cholesterol, highefficiency lipids, very low-efficiency lipids, and low-efficiency lipids, compared to healthy groups. Significant differences were observed between the study groups in the SIRP-a protein levels, as an increase in the SIRP-a concentration was observed in type 1 diabetes (15.77±7.98) compared to type 2 (12.90±6.86) and the control group (8.53±5.18). Relative gene expression results show down regulation of SIRP-a gene expression in diabetic patients (T1D and T2D) compared with control group indicative of enhanced immune response in diabetes. This study concludes that both protein levels and gene expression of SIRP-a are altered in diabetes which might shed a light in contributing this marker in the immunotherapy control of diabetes. [ABSTRACT FROM AUTHOR] |
