| Autor: |
J., Georgia Benitha, Sagar, Sandra, Jeyaraj, Genickson, Ohri, Tanvi, Shirlal, Shilpa, Sandhu, Harleel, Kaur, Ramandeep |
| Zdroj: |
African Journal of Biomedical Research; Sep2024, Vol. 27 Issue 3, p1035-1042, 8p |
| Abstrakt: |
Background: Nanotechnology is focused on the development of nanoparticles of various sizes and chemical compositions as well as their use in health research for human benefits. Selenium nanoparticles (SeNPs) are well-known bioactive compounds. Selenium nanoparticles are synthesized by various chemical and biological methods. We have formulated Euphorbia tirucalli-mediated selenium nanoparticles and studied their antimicrobial and cytotoxic activities. Aim: This study aims to formulate a selenium-mediated aqueous extract of Euphorbia tirucalli and to evaluate the antimicrobial activity and cytotoxicity against oral pathogens. Materials and methods: 0.5 mg of Sodium selenite in distilled water was added to 40 ml of Euphorbia tirucalli plant extract. The preparation of the selenium-mediated aqueous extract was examined by UV Vis - Spectrometry at the wavelength of 300-650 nm. Further evaluation for its antimicrobial activity was performed by measuring the zone of inhibition against oral pathogens. By using Brine Shrimp Lethality Assay (BSLA), the cytotoxicity was determined. Results: The selenium-mediated aqueous Euphorbia tirucalli extract characterization showed maximum absorbance at 525 nm. The Agar well diffusion method showed the maximum ZOI in Staphylococcus aureus at 18mm at 50 ug/ml and in Lactobacillus at 18 mm at 50 ug/ml. Using the BSLA method, the cytotoxicity increased with increasing concentration of the extract, showing 10 alive nauplii at 5ul and 8 alive nauplii at 80ul. Conclusion: The green synthesis of selenium-mediated Euphorbia tirucalli showed a potent antimicrobial activity and reduced lethality. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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