| Autor: |
Ponnusamy, Kalaiselvi, Vadive, Subash, Manickam, Chitra, Kumar, Praveenraj, Natesan, Senthilkumar |
| Předmět: |
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| Zdroj: |
African Journal of Biomedical Research; Sep2024, Vol. 27 Issue 3, p349-356, 8p |
| Abstrakt: |
A method for estimating Spironolactone and Hydrochlorothiazide in both bulk and tablet dose forms simultaneously was devised and validated using a stability-indicating High-Performance Thin-Layer Chromatography (HPTLC) methodology. Using a methanol mobile phase in a 3:7 ratio, the chromatographic separation was accomplished on a silica gel 60 F254 plate. The detecting procedure made use of a wavelength of 270 nm. The method showed remarkable linearity for Spironolactone and Hydrochlorothiazide over concentration ranges of 100-600 µg/mL and 50-300 µg/mL, respectively, with correlation coefficients (r²) of 0.999. Results from precision studies showed that the approach is reproducible, with intra-day RSDs of 0.81% for Spironolactone and 0.92% for Hydrochlorothiazide and inter-day RSDs of 1.09% for Spironolactone and 1.15% for Hydrochlorothiazide. Accuracy was confirmed through recovery studies with values of 98.6% for Spironolactone and 99.2% for Hydrochlorothiazide. The method also effectively separated both drugs from their degradation products, ensuring their applicability for stability testing. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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