| Autor: |
Kim-Yip, Rebecca P., McNulty, Ryan, Joyce, Bradley, Mollica, Antonio, Chen, Peter J., Ravisankar, Purnima, Law, Benjamin K., Liu, David R., Toettcher, Jared E., Ivakine, Evgueni A., Posfai, Eszter, Adamson, Britt |
| Zdroj: |
Nature Biotechnology; Dec2024, Vol. 42 Issue 12, p1822-1830, 9p |
| Abstrakt: |
Using transient inhibition of DNA mismatch repair during a permissive stage of development, we demonstrate highly efficient prime editing of mouse embryos with few unwanted, local byproducts (average 58% precise edit frequency, 0.5% on-target error frequency across 13 substitution edits at 8 sites), enabling same-generation phenotyping of founders. Whole-genome sequencing reveals that mismatch repair inhibition increases off-target indels at low-complexity regions in the genome without any obvious phenotype in mice. Prime editing is used to efficiently edit early-stage mouse embryos with few on-target byproducts. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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