| Autor: |
Mazigh, Doha, Zanane, Chorouk, El Othmany, Rabha, Mitro, Soukaina, Hakim, Taoufik, Lekchiri, Souad, El Louali, Mostafa, Latrache, Hassan, Zahir, Hafida |
| Předmět: |
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| Zdroj: |
Malaysian Journal of Microbiology; Oct2024, Vol. 20 Issue 5, p595-605, 11p |
| Abstrakt: |
Aims: Streptomyces is a soil-dwelling species that undergo complex structural and metabolic differentiation during its life cycle. As the source of a plethora of naturally bioactive molecules, Streptomyces have been successfully used in plant growth promotion and biotechnology. However, even though these bacteria are fixed in nature, little is known about them. The aim of this study was to advance the comprehension of Streptomyces biofilm production by monitoring and evaluating the kinetics of biofilm formation in Streptomyces strains over a 21-day period. Methodology and results: Six Streptomyces species were tested for their ability to produce biofilms using phenotypic methods, including microtiter plates (MTP) and the Congo red agar (CRA). Quantitative assessment of the biomass attached by MTP showed that five out of the six Streptomyces strains studied S. albogriseolus (S81), S. albogriseolus (S65), S. rochei (S11), S. lilaceus (S30) and S. griseorubens (S23) - produced robust biofilms. Additionally, the production evolution over incubation time exhibited a non-linear trend (p<0.05). In contrast, only S. rochei (S11) was identified by CRA as forming a biofilm through slime production. Furthermore, the black slime-indicating colouration displayed instability, lasting only three days under monitoring. Conclusion, significance and impact of study: Considering the lengthy life cycle of Streptomyces and the unique attributes of microtiter plate and Congo red agar techniques, the synergy between MTPs and CRA enriches the capacity for quantifying, monitoring and characterizing Streptomyces biofilms effectively. This integrated approach fosters a deeper understanding of their behaviour and provides valuable insights for practical applications. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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