| Autor: |
RAHMAN, Mohammad Mahbubur, HARUN-OR-RASHID, Md., ISLAM, Md. Ashraful, KABIR, Md. Humayun, MEAH, M. Bahadur |
| Předmět: |
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| Zdroj: |
Annals of Oradea University, Biology Fascicle / Analele Universităţii din Oradea, Fascicula Biologie; 2024, Vol. 31 Issue 1, p61-66, 6p |
| Abstrakt: |
To choose healthy seeds from a seed lot, an experiment was done to determine whether and how much wheat seeds had the blast pathogen. Total 145 wheat seed samples were gathered from three upazilas in Bangladesh's Meherpur district in the winter of 2019-Meherpur Sadar, Mujibnagar, and Gangni. Seeds were checked for any seed infection using a stereo-binocular microscope while incubated in moist blotter paper at room temperature (25°C±1). Magnaporthe oryzae pathotype Triticum (MoT) growth was confirmed by pathological analysis for any growth that was visible under stereo-binocular. 35 samples out of 145 were infected with MoT, with severity ranging from 2-16%. A PCR was conducted using the primers MoT3 and Pot2a on fungal isolates from seeds with varying amounts of MoT infection (0, 5, 10, and 20%). Monomorphic bands of 350 bp and 361 bp were obtained by agarose (1.5%) gel electrophoresis. This proved the seed infection was caused by Magnaporthe oryzae pathotype Triticum, as seen in the moist blotter. MoT3 and Pot2a primer were used to analyze 12 samples, finding 10 infected wheat samples, 1 seemingly healthy wheat seed sample, and 1 infected rice blast sample. According to the findings, 6 samples-including the one that was sampled and appeared to be in good health-clearly amplified bands of 350 bp by MoT3 primer, and seven samples amplified bands of 361 bp by Pot2a primer. This study's findings show that the moist blotter technique effectively detects MoT presence in wheat seeds, offering farmers a tool to determine seed suitability for planting. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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