| Autor: |
Schmidt, Michael, Weis, Christina, Heck, Julia, Montag, Thomas, Nicol, Sven-Boris, Hourfar, Michael K., Schaefer, Volker, Sireis, Walid, Roth, W. Kurt, Seifried, Erhard |
| Předmět: |
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| Zdroj: |
Vox Sanguinis; Oct2005, Vol. 89 Issue 3, p135-139, 5p |
| Abstrakt: |
Background and Objectives The prevention and detection of bacterial contamination of platelet concentrates remains a major challenge for transfusion medicine. To be suitable for blood-transfusion services, the contamination detection method must be highly sensitive, easy to perform and preferably of low cost. In this spiking study, we evaluated the new optimized Scansystem™ Platelet Kit detection method for use on apheresis platelets. Study Design and Methods Apheresis platelet concentrates (APCs) were individually spiked with 10 colony-forming units (CFU)/ml of one of 10 different strains of bacteria. The spiked APCs were analysed at specific time-points during incubation by using the optimized Scansystem™ Platelet Kit. Bacterial enumeration was performed by plating onto blood agar. Results All the bacterial strains tested were detected by using the optimized Scansystem™ Platelet Kit when sampled 24 h after spiking. Compared to the Scansystem™ standard kit, sensitivity was increased to < 50 CFU/ml. The identity of the spiked bacteria was confirmed by Gram staining and DNA fingerprinting. Conclusion The optimized Scansystem™ Platelet Kit was able to reliably detect, within 70 min, 10 transfusion-relevant bacterial species in APCs when a sample volume was taken 24 h after spiking. This is the first study carried out by using the optimized Scansystem™ bacterial detection that was found to have an enhanced sensitivity compared to the standard kit. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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