Establishment of a method to measure the intracellular potassium ion concentration of brain tissue using a simple device.

Autor: Iwamoto, Takaya, Fujita, Minori, Futamata, Yukiko, Okada, Teruki, Morinaga, Ryuta, Nishi, Airi, Kinjo, Toshihiko, Kawada, Koichi, Uno, Kyosuke, Kuramoto, Nobuyuki
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Zdroj: AIMS Neuroscience; 2024, Vol. 11 Issue 3, p1-14, 14p
Abstrakt: Intracellular potassium ion (K+) concentration is higher than extracellular K+ concentration. Some cells maintain intracellular potassium levels by taking up extracellular potassium. However, investigating these details requires techniques to measure intracellular potassium concentrations. We established a method to easily measure intracellular potassium concentrations using a simpler electrode. The LAQUAtwin K-11 was capable of linearly quantifying potassium concentrations and was not affected by cellular constituents other than nucleic acids; however, it did not tolerate low temperatures. Interference caused by a high concentration of nucleic acids was eliminated by the addition of cations. It was also suggested that the concentration of nucleic acids in the cell suspension was not sufficiently high to interfere with the measurements. Intracellular potassium concentrations increased and decreased in response to extracellular potassium concentrations. Exposure to valinomycin did not decrease the potassium concentration, suggesting that re-uptake of the potassium released outside the cells occurred immediately. Additionally, potassium concentrations could be measured in the brain tissue homogenates using the device. This measurement method can track the relative changes in potassium concentration in cells under various conditions and in tissues of various disease models. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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