| Autor: |
Essington, Erin A., Vezeau, Grace E., Cetnar, Daniel P., Grandinette, Emily, Bell, Terrence H., Salis, Howard M. |
| Předmět: |
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| Zdroj: |
Nature Communications; 12/2/2024, Vol. 15 Issue 1, p1-12, 12p |
| Abstrakt: |
Microbes can be engineered to sense target chemicals for environmental and geospatial detection. However, when engineered microbes operate in real-world environments, it remains unclear how competition with natural microbes affect their performance over long time periods. Here, we engineer sensors and memory-storing genetic circuits inside the soil bacterium Bacillus subtilis to sense the TNT explosive and maintain a long-term response, using predictive models to design riboswitch sensors, tune transcription rates, and improve the genetic circuit's dynamic range. We characterize the autonomous microbial sensor's ability to detect TNT in a natural soil system, measuring single-cell and population-level behavior over a 28-day period. The autonomous microbial sensor activates its response by 14-fold when exposed to low TNT concentrations and maintains stable activation for over 21 days, exhibiting exponential decay dynamics at the population-level with a half-life of about 5 days. Overall, we show that autonomous microbial sensors can carry out long-term detection of an important chemical in natural soil with competitive growth dynamics serving as additional biocontainment. Engineered microbes can detect harmful chemicals, but may not work well in complex environments. Here, the authors built microbial sensors for detection of TNT explosive and tested their response over 28 days in contaminated soil with many natural microbes, achieving stable detection for 21 days. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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