| Autor: |
Lopes, Ricardo, Sampaio, Filipe, Carvalho, Hugo Lima de, Garcês, Andreia, Fernandes, Cátia, Neves, Carolina Vitória, Brito, Alexandre Sardinha de, Marques, Tiago, Sousa, Carlos, Silva, Ana Rita, Martins, Ângela, Cardoso, Luís, Coelho, Ana Cláudia, Duarte, Elsa Leclerc |
| Předmět: |
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| Zdroj: |
Veterinary Sciences; Nov2024, Vol. 11 Issue 11, p563, 25p |
| Abstrakt: |
Simple Summary: Feline infectious peritonitis (FIP) is a severe and even fatal inflammatory disease of cats, caused by feline coronavirus (FCoV), and remains an important diagnostic challenge. This study validates the flow cytometry-based delta total nucleated cells (∆TNC) on the Sysmex XN-1000V® in effusion samples and introduces the FIP Effusion Index, a novel diagnostic method that integrates cellular and inflammatory markers of FIP in effusions. The FIP Effusion Index demonstrated improved diagnostic accuracy, achieving 96.3% sensitivity and 95.7% specificity for values of 5.06 or higher, and perfect specificity (100%) with 96.3% sensitivity at values of 7.54 or above. This combined approach outperforms traditional methods, providing a superior, rapid, and cost-effective tool for the accurate and timely FIP diagnosis. It offers considerable potential for enhancing the management of suspected FIP cases in clinical practice, with potential applications in both veterinary and human medicine for related coronavirus diseases. The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led the medical and scientific community to explore the pathogenesis and clinical manifestations of coronaviruses. In felines, a widespread coronavirus known as feline coronavirus (FCoV) can lead to feline infectious peritonitis (FIP), a highly fatal disease characterised by severe systemic inflammation. Diagnosing FCoV remains challenging due to the limited accuracy of the available methods. The present study introduces the FIP Effusion Index, a novel diagnostic method that combines the albumin-to-globulin (ALB/GLOB) ratio with the delta total nucleated cell (∆TNC) count obtained via flow cytometry using the Sysmex XN-1000V® analyser in effusions. Samples from cats (n = 50) with suspected FIP were analysed for ∆TNC, with findings showing that a ∆TNC ≥ 2.1 is highly indicative of FIP and a ∆TNC ≥ 4.9 can be considered diagnostic. The FIP Effusion Index enhanced diagnostic precision in our group of samples, achieving 96.3% sensitivity and 95.7% specificity for values ≥ 5.06, and reaching perfect specificity (100%) with 96.3% sensitivity for values ≥ 7.54. This combined approach surpasses the accuracy of individual parameters, establishing the FIP Effusion Index as a superior diagnostic tool for FIP, with potential applications in both veterinary and human medicine for related coronavirus diseases. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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