| Autor: |
Cui, Haiyue, Yang, Huan, Qi, Xiaoxi, Zhao, Yang, Huang, Tianle, Miao, Liguang |
| Předmět: |
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| Zdroj: |
Veterinary Sciences; Nov2024, Vol. 11 Issue 11, p545, 15p |
| Abstrakt: |
Simple Summary: A novel peptide derived from bovine lactoferrin has been developed by artificially combining functional fragments with antimicrobial and immunomodulatory activities. This study aimed to investigate the activating effect of this peptide on mouse immune cells and its immune-enhancing impact on the intestine. The results showed that the peptide enhanced macrophage phagocytosis and increased IL-1β mRNA production. Additionally, it regulated the expression of IL-6 and IL-10 mRNA in the intestinal tract in a dose-dependent manner, contributing to the modulation of intestinal immunity. We hope that this study will provide a new reference program for the treatment of animal diseases. Bovine lactoferrin is a natural iron-binding glycoprotein known for its antimicrobial, antiviral, antitumor, anti-inflammatory, and immunomodulatory properties. In this study, we artificially recombined a fragment of bovine lactoferrin with immunomodulatory and antimicrobial properties to create a novel peptide named LF-MQL. The primary objective was to investigate the effects of LF-MQL on the intestinal tract and immune cells in animals. First, we assessed the in vitro activation effects of LF-MQL on mouse peritoneal macrophages. The results indicated that LF-MQL enhanced the macrophage phagocytic activity and increased IL-1β mRNA expression without significantly affecting IL-6 mRNA levels. Next, we examined the effects of LF-MQL on mucosal immunity by administering LF-MQL orally at doses of 300 mg/kg, 30 mg/kg, and 3 mg/kg to mice. The results demonstrated that different doses of LF-MQL modulated IL-6 and IL-10 mRNA levels in the small intestine. Low doses enhanced the intestinal immune response, while higher doses reduced the inflammatory response. In conclusion, LF-MQL exerts immunomodulatory effects rather than simply boosting immune activity in animal models. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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