| Autor: |
Wang, Xiaoquan, Yao, Yao, Yang, Wenhao, Lu, Xiaolong, Gao, Ruyi, Liu, Kaituo, Chen, Yu, Gu, Min, Hu, Jiao, Hu, Shunlin, Liu, Xiufan, Liu, Xiaowen |
| Předmět: |
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| Zdroj: |
Veterinary Sciences; Nov2024, Vol. 11 Issue 11, p532, 17p |
| Abstrakt: |
Simple Summary: Newcastle disease is a devastating virus that affects poultry, causing illness, death, and significant economic losses. Current live vaccines struggle to protect against the most common type, genotype VII, and can be hindered by maternal antibodies. We have developed a new vaccine called LX-OAI4S by combining two strains with different genotypes, bringing together the advantages of both strains. This hybrid vaccine offers strong protection against genotype VII Newcastle disease and works well even when chickens have maternal antibodies. Importantly, it can be easily administered through a spray, making it a promising tool for poultry farmers to prevent outbreaks and protect their flocks. Newcastle disease virus (NDV) poses a significant threat to the poultry industry, with the emergence of genotype VII NDV leading to extensive outbreaks and economic losses. Vaccination is the primary means of controlling NDV, but the presence of maternal antibodies (MDAs) can interfere with the immunological effect of live virus vaccines. Thus, we constructed a chimeric NDV live virus vaccine, LX-OAI4S, by replacing the extracellular regions of the F and HN genes of the NDV LX strain with the corresponding regions of the A-VII vaccine strain. The chimeric vaccine LX-OAI4S demonstrated high genetic stability, good safety, and strong reproductive capacity in chicken embryos. The LX-OAI4S vaccine induced rapid antibody production in specific pathogen-free (SPF) and commercial chickens via the intranasal and intraocular (IN/IO) routes, with hemagglutination inhibition (HI) antibody titers reaching 4.71 ± 1.03 log2 at 7 days post-vaccination (dpv), significantly higher than those of the two classical vaccine strains La Sota and VG/GA. The LX-OAI4S vaccine group provided effective protection against the challenge of genotype VII NDV virulent strain JS2/06 and inhibited viral shedding. When administered via spray, the LX-OAI4S vaccine elicited high systemic immunity against NDV in both SPF and commercial chickens, effectively protecting against clinical disease and reducing viral shedding. The chickens were exposed to high-dose vaccine for spray vaccination, and no adverse reactions were observed after vaccination. Despite the presence of anti-NDV MDAs in chickens, the NDV-specific antibody titers were significantly greater in the vaccinated groups than in the unvaccinated group. The vaccine exhibited high immunogenicity and the potential to overcome maternal antibody interference. The LX-OAI4S vaccine is a promising candidate for an ND vaccine. Its administration via spray can effectively prevent the occurrence of ND, making it a valuable tool for the poultry industry. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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