| Autor: |
Toscano, Giorgia, Rosati, Martina, Barbieri, Letizia, Maier, Katharina, Banci, Lucia, Luchinat, Enrico, Konrat, Robert, Lichtenecker, Roman J. |
| Předmět: |
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| Zdroj: |
Chemical Communications; 12/14/2024, Vol. 60 Issue 96, p14188-14191, 4p |
| Abstrakt: |
19F nuclei serve as versatile sensors for detecting protein interactions and dynamics in biomolecular NMR spectroscopy. Although various methods have been developed to incorporate fluorine-containing aromatic residues into proteins using E. coli or cell-free expression techniques, similar approaches for protein production in mammalian cell lines remain limited. Here, we present a cost-effective synthetic route to obtain selectively deuterated, carbon-13 labeled fluorotryptophan and demonstrate its use in introducing 19F–13C spin pairs into carbonic anhydrase 2 and superoxide dismutase, following an expression protocol utilizing HEK cells. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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