Laccase-catalyzed tyrosine click reaction with 1-methyl-4-arylurazole: rapid labeling on protein surfaces.

Autor: Nakane, Keita, Fujimura, Chizu, Miyano, Shogo, Liu, Zhengyi, Niwa, Tatsuya, Nishi, Hafumi, Kadonosono, Tetsuya, Taguchi, Hideki, Tomoshige, Shusuke, Ishikawa, Minoru, Sato, Shinichi
Předmět:
Zdroj: Chemical Communications; 12/14/2024, Vol. 60 Issue 96, p14208-14211, 4p
Abstrakt: Our study demonstrates the exceptional efficiency of 1-methyl-4-arylurazole (MAUra) for tyrosine labeling, optimized with laccase under mild conditions, achieving a high efficiency (kcat/Km = 7.88 × 104 M−1 s−1) with minimal oxidative side reactions and selective labeling of highly exposed tyrosine sites on proteins. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index