Effects of Genetic Polymorphism in the IFI27 Gene on Milk Fat Traits and Relevance to Lipid Metabolism in Bovine Mammary Epithelial Cells.

Autor: Jiang, Xinyi, Zhao, Zhihui, Chen, Xuanxu, Miao, Fengshuai, Li, Jing, Yu, Haibin, Jiang, Ping, Lin, Ziwei
Předmět:
Zdroj: Animals (2076-2615); Nov2024, Vol. 14 Issue 22, p3284, 12p
Abstrakt: Simple Summary: In this study, six SNPs (UTR-(-127) C>A, UTR-(-105) T>A, UTR-(-87) G>A, I1-763 G>T, E2-77 G>A, E2-127 G>T) were detected in the IFI27 gene in a Chinese Holstein cow population. In addition, association analysis of the polymorphism of IFI27 and milk quality traits showed that the AG and GG genotypes of E2-77 G>A, and the GG and the TT genotypes of E2-127 G>T were connected to milk fat. Haplotype frequency analysis showed that individuals with an H5H6 genotype produced higher milk fat content than these with an H5H5 haplotype combination. Moreover, overexpression of the IFI27 gene in bovine mammary epithelial cells caused a significant increase in triglycerides content and a decrease in cholesterol and nonestesterified fatty acid content, while interference with IFI27 expression produced opposing changes. These results provide reference for the selection of milk fat traits in dairy cattle breeding and lay the foundation for further research of IFI27 gene function in milk fat metabolism. Milk fat is an important indicator for evaluating milk quality and a symbol of the core competitiveness of the dairy industry. It can be improved through genetic and feed management factors. Interferon alpha-inducible protein 27 (IFI27) was found to be differentially expressed when comparing the transcriptome in high- and low-fat bovine mammary epithelial cells (bMECs) in our previous research. Therefore, this study aimed to investigate whether the IFI27 gene had a regulatory effect on lipid metabolism.We detected six SNPs in the IFI27 gene (UTR-(-127) C>A, UTR-(-105) T>A, UTR-(-87) G>A, I1-763 G>T, E2-77 G>A, E2-127 G>T) in a Chinese Holstein cow population. Association analysis of the polymorphism of IFI27 and milk quality traits showed that the AG and GG genotype of E2-77 G>A, and the GG and TT genotypes of E2-127 G>T were connected to milk fat (p < 0.05). Haplotype frequency analysis showed that H5H5 was associated with lower milk fat content (p < 0.05), while milk from H5H6 animals had a higher fat content (p < 0.05). Subsequently, IFI27 overexpression vectors (PBI-CMV3-IFI27) and interference vectors (Pb7sk-GFP-shRNA) were constructed. Overexpression of the IFI27 gene in bMECs caused a significant increase in triglycerides (TGs) content (p < 0.05) and decreases in cholesterol (CHOL) and nonestesterified fatty acid (NEFA) content (p < 0.05), while interference with IFI27 expression produced opposing changes (p < 0.05). In summary, IFI27 E2-77 G>A and IFI27 E2-127 G>T may be useful as molecular markers in dairy cattle to measure milk fat, and the IFI27 gene may play an important role in milk lipid metabolism. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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