| Abstrakt: |
Simple Summary: Boar sperm demonstrate significant sensitivity to the conditions during cryopreservation, with various factors, and show a reduction in their fertilization capacity due to damaging effects on sperm cell membranes. We investigated the effects of crude garden cress seed oil (CGCSO) on sperm viability, motility, acrosome integrity, mitochondrial function, and antioxidant activity in frozen boar semen. CGCSO improved post-thawed semen qualities such as the total motility, progressive motility, viability, acrosome integrity, and mitochondrial function as well as reduced lipid peroxidation. As a result, we suggested that 1% CGCSO in the freezing extender diminished the oxidative damage caused by cryopreservation and led to improved sperm quality after thawing. This study aimed to examine the effects of crude garden cress seed oil (CGCSO) on frozen–thawed boar sperm qualities. Semen ejaculates (n = 12) were collected and further divided into six equal aliquots based on CGCSO concentrations (0, 0.5, 1, 1.5, 2, and 2.5% v/v) in the freezing extender. Semen samples were processed and cryopreserved utilizing the traditional liquid nitrogen vapor technique. Subsequently, semen samples were thawed in a thermos with warm water at 50 °C for 12 s and evaluated for sperm morphology using scanning electron microscopy, sperm motility using a CASA, sperm viability, acrosome integrity, mitochondrial function, MDA level, total antioxidant capacity (TAC), glutathione peroxidase (GSH-Px), and catalase (CAT) activity. The results indicated that 1% CGCSO resulted in superior post-thaw sperm characteristics, including enhanced sperm morphology, motility, viability, acrosome integrity, and mitochondrial function. Particularly, the total motile sperm increased by 16.5%, progressive motile sperm increased by 13.0%, viability improved by 15.1%, acrosome integrity increased by 14%, and mitochondrial function improved by 14.1% compared to the control group. CGCSO treatment at 1% and 1.5% exhibited the lowest level of MDA (45.73 ± 11.2 and 45.73 ± 11.3 µmol/L, respectively) compared to the other groups. The CGCSO-supplemented groups showed higher values of TAC, GSH-Px, and CAT than the control group but not significantly. [ABSTRACT FROM AUTHOR] |
