| Abstrakt: |
Considering the growing environmental concerns, bioutilization of keratin waste with keratinase has emerged as a potential solution to alleviate the significant burden of poultry processing waste. The main objectives of the study were to isolate and screen keratinase-producing bacteria and to optimize the production conditions of the enzyme using a statistical approach. A promising bacterium, Bacillus subtilis GH2, was isolated from a traditional leather tanning pond. This bacterium was identified using 16 S rDNA gene sequencing. By optimizing the medium components and culture conditions, a significant increase in keratinase activity of 421.65 U was obtained at a medium pH of 7.5 and temperature of 38 °C. This corresponds to a 1.96-fold increase compared to the first screening stage. The optimal activity of the enzyme was obtained at a pH of 7 and a temperature of 45 °C. Ca+2 showed the highest improved keratinase activity. Furthermore, the bacterium showed promising potential for feather biodegradation. [ABSTRACT FROM AUTHOR] |
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