| Autor: |
Perera, H. K. I., Jayasinghe, J. A. V. R., Lankarathna, A. P. C. P., Nilmanel, K. G., Sivakanesan, R. |
| Předmět: |
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| Zdroj: |
Ceylon Journal of Science; 2024, Vol. 53 Issue 3, p389-397, 10p |
| Abstrakt: |
Glycation induced protein damage, and oxidative stress play a critical role in the development of chronic diabetic complications and skin aging. Terminalia arjuna (Kumbuk) bark has been used for medicinal purposes for thousands of years. Main objectives of the study were to assess in vitro inhibitory effects of T. arjuna bark extracts on glycation induced protein cross-linking and to assess heat stability of those effects. Additional objectives were to assess antityrosinase effects, total antioxidant activity, phenolic content, and flavonoid content of T. arjuna extracts. The effects of T. arjuna bark extracts were assessed using electrophoresis-based and colorimetric methods. Experiments were repeated on three separate occasions with appropriate controls. Fiji/ImageJ software was used to quantify the extent of protein cross-linking. Statistical significance of the differences of colorimetric assays was calculated using ANOVA. P<0.05 was considered statistically significant. T. arjuna extracts showed strong inhibitory effects on glycation induced protein cross-linking and on tyrosinase activity. These effects showed heat stability to a great extent. Methanol extract demonstrated higher inhibitory effects on protein cross-linking compared to that of ethanol extracts. T. arjuna extracts demonstrated high total antioxidant capacity, total phenolic and flavonoid content. Furthermore, antioxidant capacity showed a significantly positive correlation with polyphenolic and flavonoid contents. The results provide evidence to suggest the beneficial effects of T. arjuna bark in alleviating some mechanisms that cause chronic diabetic complications and skin aging. Findings also provide in vitro evidence to validate the traditional use of T. arjuna bark in diabetes mellitus and skin disorders. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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