| Abstrakt: |
The objective of this study was to assess the biological activities of Anthemis cotula L. and explore its potential therapeutic effects on A549 lung cancer cells. Within this particular framework, the aerial components of the plant were collected and subjected to extraction using a Soxhlet apparatus to obtain extracts in the form of water, ethanol, and methanol. The determination of the phenolic content of the samples was conducted using the Folin-Ciocalteu reagent, while the quantification of flavonoid content was conducted via an aluminum chloride assay, and the measurement of the plant's total antioxidant, total oxidant, and oxidative stress index was conducted using Rel Assay kits. The antimicrobial properties of the substances were examined via the agar dilution technique. The MTT assay was employed to determine the antiproliferative activity against A549 lung cancer cells, and the LC-MS/MS instrument was utilised to screen for the presence of standard compounds. The following compounds were identified in the study: acetohydroxamic acid, catechin hydrate, vanillic acid, resveratrol, kaempferol, fumaric acid, gallic acid, protocatechuic acid, 4-hydroxybenzoic acid, caffeic acid, salicylic acid, 2-hydroxycinnamic acid, naringenin, quercetin, luteolin, and alizarin. Plant extracts were found to be effective against microorganisms in the range of 25 to 200 μg/mL; furthermore, the water extract exhibited the highest total antioxidant status value of 8.201 ± 0.113 μmol/L. Similarly, the ethanol extract demonstrated the highest total oxidant status value of 11.247 ± 0.178 μmol/L, while, lastly, the methanol extract displayed the highest oxidative stress index value of 0.151 ± 0.001. Strong antiproliferative activity was observed in all the plant extracts, and the ethanol extract with the highest concentration exhibited a total phenolic content of 61.78 ± 4.38 mg/g. The ethanol extract exhibited the highest recorded total flavonoid content, measuring 66.84 ± 3.13 mg/g. In the present context, it has been observed that the plant demonstrates significant biological activities; consequently, A. cotula is believed to possess significant potential as a valuable resource in the development of pharmacological designs. [ABSTRACT FROM AUTHOR] |
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