| Abstrakt: |
The occurrence of pharmaceuticals in the environment can have undesirable effects on nontarget animals, including fish. The present experiment assessed the effects of subchronic exposure to waterborne acetaminophen (N-acetyl-p-aminophenol) (APAP) on selected behavioral aspects (physical avoidance response, ventilation rate, and food detection rate) and erythrocyte nuclear abnormality (ENA) in juvenile Oreochromis niloticus. Two groups of fish were exposed to APAP dissolved in aged municipal water (T1: 2 mg/L and T2: 10 mg/L) for 8 weeks in three replicates (n = 7 fish per tank), alongside a control group (C) without APAP. APAP-exposed fish spent significantly (p <.05) longer time to detect food (T1: 32.6 ± 4.55 s and T2: 39.6 ± 4.66 s) compared to the control group (19.9 ± 2.46 s). Both APAP-exposed groups exhibited attenuated physical avoidance responses (76.7%, 68.7%, and 87.3% in T1, T2, and C, respectively) and a lower mean ventilation rate compared to the control group (194.5 ± 15.5, 179.1 ± 11.6, and 233.2 ± 19.0 per min in T1, T2, and C, respectively). The frequency occurrence of ENA types such as bi-nucleated, notched nuclei, lobed nuclei, and blebbed nuclei (except micronuclei) was significantly higher (p <.05) in APAP-exposed groups compared to the control, with more pronounced effects in the T2 group. The study concludes that APAP exposure prompts significant alterations in behavior and erythrocyte nuclear morphology, emphasizing the value of monitoring and regulating the entry of pharmaceuticals, including APAP, into aquatic environments to prevent unintended effects on non-target organisms like Oreochromis niloticus. [ABSTRACT FROM AUTHOR] |
