| Abstrakt: |
Introduction: Due to the concerning increase in resistance to traditional antimicrobial drugs, new therapies to fight antibiotic-resistant microorganisms, such as plant-based compounds and nanoparticles, appear necessary. The goal of this research was to examine the antimicrobial activity of silver nanoparticles synthesized using Lycium ruthenicum extract. Methods & Materials: In this experimental study, Escherichia coli (ATCC25922), Pseudomonas aeruginosa (ATCC27853), Klebsiella pneumoniae (ATCC9997), Staphylococcus aureus (ATCC25923) and Enterococcus faecalis (ATCC29212) were studied. Aqueous extract of L. ruthenicum was prepared. Silver nanoparticles were prepared in combination with L. ruthenicumextract. The MIC of silver nanoparticles was investigated alone and in combination with the L. ruthenicumplant using the broth microdilution method for Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus and Enterococcus faecalis. Results: The nanoparticle synthesized with the aqueous extract of the Lycium ruthenicum plant had a significant inhibitory effect on E. coli, P. aeruginosa, and S. aureus compared to the aqueous extract alone (P<0.05). Also, the highest inhibitory activity of L. ruthenicum extract was for K.pneumoniae and E. faecalis with MIC 2.5 mg / ml. On the other hand, biosynthesized silver nanoparticles against E. coli with MIC 1.25 mg / ml showed the highest inhibitory activity. In general, silver nanoparticles synthesized with L. ruthenicum extract had significant antibacterial effects on the studied bacteria, in comparison with silver nanoparticles by chemical method as well as the plant L. ruthenicum alone. Conclusion: Silver nanoparticles synthesized with L. ruthenicum extract have significant antibacterial effects, which can be used as a suitable treatment option for antibiotics due to the side effects of antibiotics after further studies on animal models. [ABSTRACT FROM AUTHOR] |
