Biosynthesis of Selenium Nanoparticles Using the Bacteria Isolated from the Glass-Making Factories Effluents.

Autor: Sardarabadi, Hadi, Zohrab, Fatemeh, Tavakolizadeh, Mahdi, Akhlaghi, Mahdi, Salarinia, Reza
Zdroj: BioNanoScience; Dec2024, Vol. 14 Issue 5, p5194-5202, 9p
Abstrakt: Selenium nanoparticles (SeNPs) are essential in biological research due to their significant theranostic applications. Consequently, the production of these particles has garnered considerable attention. Given the limitations of physical and chemical synthesis methods for various nanostructures, current research is primarily focused on developing safe, cost-effective, and environmentally friendly synthesis approaches. Recognizing the advantages of biological synthesis methods, a method involving the dilution of effluents from a glass-making factory and the incubation of SeNPs-producing bacteria on selenium dioxide plates was employed. Colonies producing a red color were cultivated in a nutrient broth culture medium to achieve a sufficient concentration for characterization. The red-colored bacterial colonies exhibiting a smooth surface and well-defined borders showcased the capacity to generate nanoparticles. Dynamic light scattering analysis revealed that the synthesized particles had a diameter of 117 ± 7.638 nm with a polydispersity index of 0.257 ± 0.005. Scanning electron microscope images indicated that the produced nanoscale particles exhibit a circular shape. Energy-dispersive spectroscopy confirmed that these particles consist of selenium. Furthermore, analysis of the 16srDNA sequence verified that the organisms responsible for production are bacteria. Notably, in addition to their intracellular synthesis capability, the isolated bacteria also demonstrated the ability to produce nanoparticles extracellularly. These findings present novel opportunities for utilizing bacteria in nanoparticle synthesis. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index