| Autor: |
Kaur, Harneet, Tyagi, Vikrant, Kumar, Jitendra, Roy, Joy K, Chugh, Vishal, Ahmed, Naseer, Dhaliwal, Harcharan Singh, Sheikh, Imran |
| Zdroj: |
Euphytica; Nov2024, Vol. 220 Issue 11, p1-19, 19p |
| Abstrakt: |
Biofortification of grain iron (Fe) and zinc (Zn) in cereals is a cost-effective method to address micronutrient deficiencies. Six metal homeostasis genes (YSL15, NAS2, FRO7, IREG, ZIP2 and VIT1) were pyramided in the wheat cultivar 'PBW343 LrYr' to enhance the content of Fe and Zn in the grain. The crosses of twenty pre-bred wheat-Aegilops genotypes with varying combinations of these genes resulted in the production of 1245 F1 seeds, out of which 680 were germinated. The selection of 103 F1 plants was achieved through the use of ITAP markers and inductively coupled plasma mass spectrometry (ICPMS) to screen for metal homeostasis genes and grain Fe and Zn content respectively. The 151 plants were selected from 103 F2 progeny and these plants were further whittled down to 83 in F3 and 76 in F4, with a final selection of 12 progeny that exhibited a variety of combinations of the six alleles. It is important to note that derivatives such as 'EU19144-500-477' and 'EU19144-506-503' exhibited high grain Fe and Zn contents without a yield penalty. The genes FRO7, IREG and ZIP2 exhibited the significant increase in grain Fe (140.61%) and Zn (122.75%) in the derivative 'EU19144-506-502'. The number of tillers, grain Fe, grain Zn and flag leaf Zn content were negatively correlated with thousand grain weight, while seeds per spike and plant height were positively correlated. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
Full text from SpringerLink