| Autor: |
Halbers, Lila P., Cole, Kyle H., Ng, Kevin K., Fuller, Erin B., Chan, Christelle E. T., Callicoatte, Chelsea, Metcalfe, Mariajose, Chen, Claire C., Barhoosh, Ahfnan A., Reid-McLaughlin, Edison, Kent, Alexandra D., Torrey, Zachary R., Steward, Oswald, Lupták, Andrej, Prescher, Jennifer A. |
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| Zdroj: |
Nature Communications; 11/18/2024, Vol. 15 Issue 1, p1-12, 12p |
| Abstrakt: |
A complete understanding of RNA biology requires methods for tracking transcripts in vivo. Common strategies rely on fluorogenic probes that are limited in sensitivity, dynamic range, and depth of interrogation, owing to their need for excitation light and tissue autofluorescence. To overcome these challenges, we report a bioluminescent platform for serial imaging of RNAs. The RNA tags are engineered to recruit light-emitting luciferase fragments (termed RNA lanterns) upon transcription. Robust photon production is observed for RNA targets both in cells and in live animals. Importantly, only a single copy of the tag is necessary for sensitive detection, in sharp contrast to fluorescent platforms requiring multiple repeats. Overall, this work provides a foundational platform for visualizing RNA dynamics from the micro to the macro scale. Studying RNA dynamics in vivo often relies on fluorogenic approaches, but these can be hampered by factors such as limited sensitivity and sample autofluorescence. Here, the authors describe an ultrasensitive platform for RNA imaging, which features RNA tags that recruit light-emitting luciferase fragments. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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