| Abstrakt: |
Pulpitis, a painful condition, often occurs in children. Interleukin 6 (IL-6) is one of the pro-inflammatory cytokines secreted by pulp fibroblasts, which play a crucial role in pulpitis. Eugenol is one of the anti-inflammatory agents that can inhibit IL-6. Eugenol has been clinically applied to pulpitis anodyne agents, but eugenol might irritate periapical tissues if used excessively for a long time. This study investigated how eugenol volume, exposure time, and their interaction affected IL-6 expression in pulpitis in Sprague-Dawley rats. The posttest-only control group consisted of 40 Sprague-Dawley rats. Rats were presented with 0.1 μL, 0.08 μL, and 0.02 μL eugenol and a negative control for 1 hour, 1 day, 3 days, 5 days, and 7 days. rats were decapitated, histological preparations were prepared, and immunohistochemical (IHC) staining was performed. On a 400-magnification microscope, ImageJ was used to calculate the average IL-6 expression area %. Data analysis was done through two-way ANOVA. The 0.08 μL eugenol volume exposure group had low IL-6 expression after 3 days, with 25,149 ± 0,82. IL-6 expression increased at 0,1 μL, 0,08 μL, and 0,02 μL volumes after 7 days compared to the negative control. The results of the present study showed that two-way ANOVA test shows significant variations (p < 0.05) in IL-6 expression based on eugenol volume and exposure time, with an interaction between the two variables. Low IL-6 expression was seen with a 0.08 μL amount of eugenol and 3-day exposure duration. [ABSTRACT FROM AUTHOR] |
