| Abstrakt: |
Methyl orange, a toxic colourant, is very resistant to decomposition once released into the environment. This azo dye has devastating effects on living things when ingested. Degrading methyl orange via biological techniques is an appropriate way to reduce environmental concerns. Research Tools and Procedures: Water treated with methyl orange requires the employment of the Bacillus Subtilis bacterium for remediation. By placing the bacterium in nutrient broth and leaving it to incubate, it can be grown. They were given these microorganisms at MO concentrations of 50, 75, 100, and 150 mgL−1. The rate of methyl orange degradation was measured by tracking the experiment for five days. We examine the rate of MO deterioration using a UV-Vis spectrophotometer. Categories and specimens Findings and Analysis: Among the tested concentrations of B. Subtilis, 150 mgL−1 resulted in the greatest rate of MO degradation, at 65.42 percent. Because the bacteria can't survive at such high concentrations, degradation ends at around 150 mgL−1. Eight participants make up the present study's sample, and the G-power value is eighty percent. At 150 mgL−1, the 2-tailed significance values for the degradation of methyl orange at 50, 75, 100, and 150 mgL−1 were 0.919, 0.571, 0.354, and 0.003, respectively. Since the p-value was less than 0.05 at this concentration, the relationship between the two groups is statistically significant. However, when considering the other concentrations (50, 75, 100 mgL−1), the p-value is greater than 0.05, thus the relationship is not significant. In sum, when it comes to removing MO from the environment, biological therapy has several advantages over physical and chemical treatment. [ABSTRACT FROM AUTHOR] |
