Use of the Puccinia sorghi haustorial transcriptome to identify and characterize AvrRp1-D recognized by the maize Rp1-D resistance protein.

Autor: Kim, Saet-Byul, Kim, Ki-Tae, In, Solhee, Jaiswal, Namrata, Lee, Gir-Won, Jung, Seungmee, Rogers, Abigail, Gómez-Trejo, Libia F., Gautam, Sujan, Helm, Matthew, Ahn, Hee-Kyung, Lee, Hye-Young, Read, Quentin D., Woo, Jongchan, Holan, Katerina L., Whitham, Steven A., Jones, Jonathan D. G., Choi, Doil, Dean, Ralph, Park, Eunsook
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Zdroj: PLoS Pathogens; 11/8/2024, Vol. 20 Issue 11, p1-25, 25p
Abstrakt: The common rust disease of maize is caused by the obligate biotrophic fungus Puccinia sorghi. The maize Rp1-D allele imparts resistance against the P. sorghi IN2 isolate by initiating a defense response that includes a rapid localized programmed cell death process, the hypersensitive response (HR). In this study, to identify AvrRp1-D from P. sorghi IN2, we employed the isolation of haustoria, facilitated by a biotin-streptavidin interaction, as a powerful approach. This method proves particularly advantageous in cases where the genome information for the fungal pathogen is unavailable, enhancing our ability to explore and understand the molecular interactions between maize and P. sorghi. The haustorial transcriptome generated through this technique, in combination with bioinformatic analyses such as SignalP and TMHMM, enabled the identification of 251 candidate effectors. We ultimately identified two closely related genes, AvrRp1-D.1 and AvrRp1-D.2, which triggered an Rp1-D-dependent defense response in Nicotiana benthamiana. AvrRp1-D-induced Rp1-D-dependent HR was further confirmed in maize protoplasts. We demonstrated that AvrRp1-D.1 interacts directly and specifically with the leucine-rich repeat (LRR) domain of Rp1-D through yeast two-hybrid assay. We also provide evidence that, in the absence of Rp1-D, AvrRp1-D.1 plays a role in suppressing the plant immune response. Our research provides valuable insights into the molecular interactions driving resistance against common rust in maize. Author summary: The common rust disease of maize is caused by the obligate biotrophic fungus Puccinia sorghi. Resistance to common rust is controlled by race-specific dominant NLR (nucleotide-binding domain and leucine-rich repeats) genes and by a variety of non-race-specific quantitative trait loci. The maize Rp1-D is a coiled-coil-NLR protein conferring race-specific resistance that includes a rapid localized programmed cell death, hypersensitive response (HR). In this study, to identify AvrRp1-D from an avirulent P. sorghi IN2, we employed the isolation of haustoria, facilitated by a biotin-streptavidin interaction, as a powerful approach. This method proves particularly advantageous in cases where the genome information for the fungal pathogen is unavailable, enhancing our ability to explore and understand the molecular interactions between maize and P. sorghi. The haustorial transcriptome is generated through this technique in combination with bioinformatic analyses. We identified two closely related genes, AvrRp1-D.1 and AvrRp1-D.2, which triggered an Rp1-D-dependent defense response in Nicotiana benthamiana. AvrRp1-D-induced Rp1-D-dependent HR was further confirmed in maize protoplasts. We demonstrated that AvrRp1-D.1 interacts directly and specifically with the leucine-rich repeat domain of Rp1-D through yeast two-hybrid assay. Our research provides valuable insights into the molecular interactions driving resistance against common rust in maize. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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