| Autor: |
Clarke, D. Nathaniel, Kane, Akshay, Perillo, Margherita, Lowe, Christopher J., Swartz, S. Zachary |
| Předmět: |
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| Zdroj: |
Development (09501991); Oct2024, Vol. 151 Issue 20, p1-6, 6p |
| Abstrakt: |
Delivering molecular tools into oocytes is essential for developmental and reproductive biology. Microinjection, the conventional method, is equipment intensive, often technically challenging and has a low yield, and is impractical in species with delicate oocytes or restricted spawning seasons. To overcome these limitations, we developed VitelloTag, a cost-effective, high-throughput system using vitellogeninderived fusion proteins to enable efficient cargo delivery via receptormediated endocytosis. We demonstrate its utility by delivering Cas9/sgRNA complexes in two distantly related species for gene knockout. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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