| Autor: |
Manshori, Fateme, Jalousian, Fateme, Hosseini, Seyed Hossein, Shayan, Parviz, Soltani, Minoo |
| Předmět: |
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| Zdroj: |
Iranian Journal of Veterinary Medicine; Oct2024, Vol. 18 Issue 4, p579-588, 9p |
| Abstrakt: |
Background: Iran is one of the endemic areas of Dirofilaria immitis. The modified Knott test is the common diagnostic test based on detecting and identifying microfilaria in blood samples. Still, the results may be compromised by D. immitis occult infection (D. immitis infection without microfilariae). Wolbachia pipientis in a dog's blood is an endosymbiont of D. immitis and an indicator of dirofilariasis. Objectives: This study aimed to investigate the occult infection of D. immitis in stray dogs in Guilan, Qazvin, and Mazandaran provinces, Iran, based on W. pipientis DNA tracking. Methods: Blood samples (n=138) were collected, and the presence of W. pipientis was evaluated by amplifying a partial sequence of the FtsZ gene (267 bp). Results: The results showed that 17.4% of the samples were positive by the modified Knott method, while 47.8% were positive by the molecular methods. The results of high-resolution melting (HRM) real-time PCR showed that the melting temperature of cox1 gene amplicons of D. immitis and Acanthocheilonema reconditum were obtained at 76.67±0.03 °C and 81.38±0.05 °C, respectively as well as it was achieved 80.30±0.04 °C for FtsZ gene of W. pipientis. The results of the present study showed that it is necessary to use molecular methods to diagnose dirofilariasis occult infection accurately. Conclusion: The HRM real-time PCR analysis is a simple post-PCR step that exploits the thermal characteristics of the amplicons to detect the occult infection of dirofilariasis based on W. pipientis DNA tracking and identification. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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