| Autor: |
Hart, Ryan G., Divya Kota, Fangjia Li, Mengdi Zhang, Ramallo, Diego, Price, Andrew J., Otterpohl, Karla L., Smith, Steve J., Dunn, Alexander R., Huising, Mark O., Jing Liu, Chandrasekar, Indra |
| Předmět: |
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| Zdroj: |
Journal of Cell Science; Oct2024, Vol. 137 Issue 20, p1-11, 11p |
| Abstrakt: |
Nonmusclemyosin II (NMII) generates cytoskeletal forces that drive cell division, embryogenesis, muscle contraction and many other cellular functions. However, at present there is no method that can directly measure the forces generated by myosins in living cells. Here, we describe a Förster resonance energy transfer (FRET)-based tension sensor that can detect myosin-associated force along the filamentous actin network. Fluorescence lifetime imaging microscopy (FLIM)-FRET measurements indicate that the forces generated by NMII isoform B (NMIIB) exhibit significant spatial and temporal heterogeneity as a function of donor lifetime and fluorophore energy exchange. These measurements provide a proxy for inferred forces that vary widely along the actin cytoskeleton. This initial report highlights the potential utility of myosin-based tension sensors in elucidating the roles of cytoskeletal contractility in a wide variety of contexts. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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