| Abstrakt: |
We have studied the main directions of age-related changes and the parameters of some enzymes involved in energy metabolism in the mitochondria of organs under the influence of adverse factors and a protein-deficient diet. To elucidate the influence of physical factors and a protein-deficient diet on the activity of Krebs cycle enzymes in rats and growing rat pups. Experimental studies were performed on 293 white female and male rats for insemination of females, weighing 180-200 g. Animals were placed in cages for fertilization of females for 10-12 days. Females - during the period of fertilization, after childbirth and lactation, were affected by high temperature and a protein-deficient diet and at the end of the above periods, the rats were slaughtered by decapitation. Mitochondria were isolated from liver tissue using 0.25 M sucrose, 25 mM EDTA, 10 mM Tris HCl, pH 7.4 as the isolation medium. In conditions of high air temperature and protein deficiency in the diet, metabolic processes in the liver are disturbed almost in all periods of the study, which is expressed in a significant decrease in the activity of dehydrogenases, respiratory enzyme and ATPase. Under the influence of unfavorable environmental factors and protein deficiency in the diet of mother rats - during pregnancy, childbirth and lactation and during the growth period of rat pups, after suckling mother's breast milk and during the transition of young animals to independent nutrition, a decrease in the intensity of redox reactions was observed. [ABSTRACT FROM AUTHOR] |
