| Autor: |
Singh, Shalini, Singh, Harikesh Kumar, Khatoon, Zubaida, Yadav, Alok, Waseem, Mohammad, Bin Abubaker, Mohammed, Husain, Hadiya |
| Předmět: |
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| Zdroj: |
Biochemical & Cellular Archives; Oct2024, Vol. 24 Issue 2, p2185-2192, 8p |
| Abstrakt: |
Fasciolosis is an important disease of livestock with increasing reports of human infection, caused by the helminth parasite Fasciola. In this study, we report a preliminary approach towards the development of immunosensors based on nanostructured porous silicon for the detection of Fasciola infection. The surface of porous silicon was biofunctionalized by the deposition of 3-aminopropyltriethoxysilane (APTS) from solution, leading to high density of amine groups covering the surface which would promote the further immobilization of biomolecules like antibodies. The antibody against bacterial collagenase IV binds with porous silicon and its variable region binds with Fasciola gigantica excretory/secretory (ES) products which were then detected by observing the apple green colour under fluorescence microscope by using fluorescein isothiocyanate (FITC) labeled bacterial collagenase IV antibody. In addition the FTIR study was also performed. The present study suggests the possibility of using porous silicon as immunosensors for F. gigantica infection detection with low cost and high sensitivity. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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