| Autor: |
Haniff, H. F. Seyed Mafiya, Sundararasu, Karuppiah, Askar Nawas, Pakeer Mohideen Mohideen, Begum, Benazir, Maheswari, Pandiaraj, Vijayasanthi, Murugan |
| Předmět: |
|
| Zdroj: |
Biochemical & Cellular Archives; Oct2024, Vol. 24 Issue 2, p1881-1886, 6p |
| Abstrakt: |
The aim of this study was identification and characterization of highly efficient Exopolysaccharide (EPS) producing bacteria in marine sediment soil. To identify specific exopolysaccharide producing bacteria, observations were collected regarding their morphological, physiological, and biochemical characteristics. The findings indicated that Halomonas sp. is the genus to which this bacterium belongs. The following elements were present (carbon, hydrogen, nitrogen and sulfur) in EPS. Further, to confirm the biological potential, the antibacterial Shigella flexneri (MTCC 1457), Proteus mirabilis (MTCC 1429), Escherichia coli (MTCC 43) and Streptococcus pneumoniae (MTCC 655) and MIC properties of exopolysaccharide. MTT assay was used to assess the effect of EPS on growth of Hep G2 (Hepatocellular carcinoma) cell line. These cells were cultured under different concentrations rate of EPS. The inhibitory concentrations (IC50 value) of the tested EPS was found to be 21.188215 µl/ml. Annexin V/PI staining confirmed the apoptosis induced by EPS in Hep G2 cells. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
