| Abstrakt: |
Introduction: Endometriosis is a chronic gynecological disease that can lead to infertility. Considering that the uterus is not sterile, the reflux of menstrual blood to the pelvis in these patients may be contaminated with bacteria and bacterial endotoxin, contributing to the development of endometriosis lesions. This study was conducted with aim to investigate the relationship between endometriosis, infertility, and uterine bacteria in women with endometriosis and infertility. Methods: This case-control study was conducted during summer to autumn of 2021 on the endometrial bacteria of 35 women with endometriosis and infertility, as well as 15 healthy women in Zeinabieh Hospital, Shiraz. Bacterial isolates were identified and analyzed using biochemical and molecular methods. Then, the chi-square test and SPSS (version 21) were utilized to explore the relationship between uterine microbiota and endometriosis and infertility. Results: The isolates obtained from the uterine endometrium of sick women included Enterococcus faecalis (31.8%), E. coli (18.2%), Streptococci pyogenes (6.8%), Klebsiella pneumonia (13.6%), Klebsiella aerogenes (9.1%), Staphylococci aureus (4.5%), Pseudomonas aeruginosa (9.1%), Staphylococci Epidermidis (6.8%) and in healthy women were Lactiplantibacillus plantarum (45%), E. coli (15%), Acinetobacter baumannii (10%), and Staphylococci epidermidis (30%). According to the results of chi-square test, there was a significant relationship between the type of bacteria (normal flora and pathogenic) and the studied groups (p<0.05). Among the bacteria isolated from sick and healthy women, E. faecalis (31.8%) and L. plantarum (45%) were found to be the dominant genera. Conclusion: The reduction of Lactobacillus species and the increase in the diversity of pathogenic bacteria species in the uterus are associated with an increased susceptibility to endometriosis and infertility. [ABSTRACT FROM AUTHOR] |
