| Autor: |
Al-attar, Huda M., Mohammad, Maeda H., Majeed, Ayeda M., Hussein, Haitham T., Ahmed, Aysar A. |
| Předmět: |
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| Zdroj: |
Iraqi Journal of Cancer & Medical Genetics; 2024 Supplement, Vol. 17, p34-34, 1p |
| Abstrakt: |
Introduction: Green synthesis of nanoparticle is considered as a promising method to synthesize a functionalized nanoparticle (NP) with no byproducts formed during the synthesis process when compared to chemical methods preparation. Compared to other traditional cancer treatment strategies, such as surgery, chemotherapy and radiotherapy, nanoparticle produced by green methods is considered as a powerful replacement to the above-mentioned treatment methods with no side effects. Aim: To determine the potential impact of GO/CeO2 nanoparticles NPs as an anti-tumor agent in biomedical field, an in vitro study. Methods: In the current study, CeO2 was synthesized using coprecipitation method. The method of preparation used is considered green due the usage of Allium Sativum extract in the synthesis of CeO2 nanoparticles. Graphene -Oxide (GO) was prepared by pulsed laser ablation in water (PLAL) which is also considered as a green method. The decoration of CeO2 on GO was achieved by mixing the GO with CeO2 nanocolloid. The as-prepared GO-CeO2 NPs was characterized using XRD, UV-visible spectrophotometer, FESEM, EDS and FTIR. For the in vitro study, nine CeO2 concentrations were used (starting from 0.98 – 250 µg/ml) as duplicate dilutions, to investigate the potential effect of CeO2 nanoparticles. The as-prepared GO/CeO2 were investigated against panel of cancer cell lines (human breast cancer cell line AMJ13, brain cancer cell line AMGM5, human oesophagus cancer cell line SK-GT-4) and compared with normal cell line REF both with/without using CeO2 in culturing. Results: The FESEM results show that the CeO2 NPs are homogeneous and nearly spherical in its shape with an average dimension of 55 nm. The FTIR analysis has proved that the as prepared CeO2 NPs are functionalized by the Allium Sativum extract. The in vitro results showed that the GO/CeO2 had significant potential impact in the high concentration 250 µg/ml at p> 0.05 against breast cancer cell line AMJ13 (with growth inhibition 51.04%) compared with other cell lines that showed less significant effect at p> 0.05 on brain cancer cell line AMGM5 (with growth inhibition 37.12%) and increased the proliferation rate on oesophagus cancer cell line SK-GT-4 and with no effects on control cell line REF during 48h of time exposure. Conclusions: According to the above results, we concluded that the as prepared CeO2 is a promising anticancer agent against breast cancer cell line that was proved to be safe on normal cell line REF. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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