| Autor: |
Qasim, Alyaa Jabbar, Abood, Alyaa Kareem, Al-Shammari, Ahmed Majeed, Zeadan, Ayat Majeed, Abbas, Sura Mouaid |
| Předmět: |
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| Zdroj: |
Iraqi Journal of Cancer & Medical Genetics; 2024 Supplement, Vol. 17, p29-29, 1p |
| Abstrakt: |
Utilizing the bacterial vaccines as a Bacterial-Based Cancer Therapy may be considered an elegant novel strategy that makes vaccines multifunctional tools that can play dual different significant roles in medicine. Most studies in this regard tested bacterial-based cancer immunotherapy, current work aims to try in vitro immuno-independent experiments to explore the anticancer activity of vaccine-derived bacterial toxoids in different cancer cell lines. Six concentrations of the DTP vaccine (5 x 1025, 4- x 10125, 5- x 10625, 6- x 10312, 7- x 107-, and 15 x 106- µg /ml) were tested against two different cancer cell lines (SKG, HC) and Rat Embryonic Fibroblast (REF) cell line as normal cells. Using the Crystal Violate cytotoxicity assay we assessed killing percentages for each of the six concentrations of toxoid, thus toxoid IC50 was calculated. Cytopathological effects such as apoptotic cells were evaluated under an inverted microscope. With significant statistical differences at a probability level lower than 0.001, our study found a toxic effect of microbial toxoid on all cancer cell lines SKG and HC, while for the normal REF cell line, this toxic effect was correlated only with the highest concentrations of toxoid. Cytological changes were observed in cancer cells treated with bacterial toxoid with insignificant effect on normal cells. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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