| Abstrakt: |
Beak and feather disease virus (BFDV), belongs to the Circoviridae family and structurally has seven open reading frames (ORFs). Only two ORF C1 and ORF V1 are more affected by genetic changes. BFDV is prone to mutations that allow the circovirus to maintain its virulence. Because there are differences in replication rates between Rep, Cap, and intergenic sequences, a complete evaluation of the entire BFDV genome sequence is of great importance to determine all recombination opportunities. In this study, among the parrots referred to veterinary clinics in Isfahan (2021-2022), 300 parrots suspected of PBFD from the Cockatiel, Rose-ringed parakeet and African grey parrot species were selected for sampling. Approximately 4 to 8 feathers were removed from each bird showing signs of disease, and total DNA was isolated from clinical samples. Among the positive cases, 5 samples were selected and their nucleotide sequence was determined using the Sanger method on PCR products. Comparing the nucleotide sequence of the samples showed that these 5 viruses are divided into 3 branches. Nucleotide sequences from Cockatiel samples and African gray parrot samples were clustered with 97% similarity and with African gray parrot and Budgerigar samples isolated in Saudi Arabia in 2017. Isolated samples of Rose-ringed parakeet showed the highest nucleotide similarity to samples isolated from Rose-ringed parakeet and other parrots in Saudi Arabia and South Africa. In fact, the nucleotide sequence similarity of samples from Southeast Asia and Oceania is less than that of samples from Asia and Africa. [ABSTRACT FROM AUTHOR] |
