Blocking IP3 Receptors with 2-APB Alters Cellular Signaling during 7-Day Soleus Unloading in Rats.

Autor: Zaripova, K. A., Bokov, R. O., Sharlo, K. A., Belova, S. P., Nemirovskaya, T. L.
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Zdroj: Journal of Evolutionary Biochemistry & Physiology; Sep2024, Vol. 60 Issue 5, p1795-1806, 12p
Abstrakt: Membrane IP3 receptors (IP3Rs) abound in the sarcoplasmic reticulum, nucleus and mitochondria of muscle fibers. We hypothesized that IP3R activation during muscle unloading may elicit a weak Ca2+ release signal, both cytosolic and nucleoplasmic, which promotes (perhaps in cooperation with other signaling cascades) the activation of transcription factors and thus leads to the expression or repression of genes associated with muscle phenotypes. Here, we tested this hypothesis by blocking IP3Rs with 2-APB (2-aminoethoxydiphenyl borate, 10 mg/kg in 5% DMSO i.p. daily) in a rat 7-day hindlimb suspension model of soleus muscle unloading. The blocking of IP3Rs prevented a decrease in the cross-sectional area of both slow and fast soleus muscle fibers and thus slowed down the development of atrophic processes in this postural muscle during 7-day hindlimb suspension. Such an effect of blocking IP3Rs during rat soleus muscle unloading may be due to preventing a decrease in ribosomal biogenesis and an increase in the expression of autophagy markers ULK-1 and IL-6. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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