| Autor: |
Ikeda, Hanako, Osakada, Fumitaka, Watanabe, Kiichi, Mizuseki, Kenji, Haraguchi, Tomoko, Miyoshi, Hiroyuki, Kamiya, Daisuke, Honda, Yoshihito, Sasai, Noriaki, Yoshimura, Nagahisa, Takahashi, Masayo, Sasai, Yoshiki |
| Předmět: |
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| Zdroj: |
Proceedings of the National Academy of Sciences of the United States of America; 8/9/2005, Vol. 102 Issue 32, p11331-11336, 6p |
| Abstrakt: |
We report directed differentiaion of retinal precursors in vitro from mouse ES cells. Six3+ rostral brain progenitors are generated by culturing ES cells under serum-free suspension conditions (SFEB culture) in the presence of Wnt and Nodal antagonists (Dkk1 and LeftyA), and subsequently steered to differentiate into Rx+ cells (16%) by treatment with activin and serum. Consistent with the characteristics of early neural retinal precursors, the induced Rx+ cells coexpress Pax6 and the mitotic marker Ki67, but not Nestin. The ES cell-derived precursors efficiently generate cells with the photoreceptor phenotype (rhodopsin+ recoverin+) when cocultured with embryonic retinal cells. Furthermore, organotypic culture studies demonstrate the selective integration and survival of ES cell-derived cells with the photoreceptor phenotype (marker expression and morphology) in the outer nuclear layer of the retina. Taken together, ES cells treated with SFEB/Dkk1/LeftyA/ serum/activin generate neural retinal precursors, which have the competence of photoreceptor differentiation. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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