| Abstrakt: |
Aims: Glioblastoma patients have a dismal prognosis, due to inevitable tumour recurrence and respond poorly to immunotherapy. Tumour‐associated microglia/macrophages (TAMs) dominate the glioblastoma tumour microenvironment and have been implicated in tumour progression and immune evasion. Early recurrent glioblastomas contain focal reactive regions with occasional fibrosis, chronic inflammation, TAMs and tumour cells. Surgical specimens from these tumours are rare and provide crucial insights into glioblastoma recurrence biology. This study aimed to characterise TAM‐ and lymphocyte phenotypes in primary vs early‐ and late‐recurrent glioblastomas. Methods: Patient‐matched primary and recurrent glioblastomas were compared between patients with early recurrences (n = 11, recurrence ≤6 months) and late recurrences (n = 12, recurrence after 12–19 months). Double‐immunofluorescence stains combining Iba1 with HLA‐DR, CD14, CD68, CD74, CD86, CD163, CD204 and CD206 along with stains for CD20, CD3, CD8 and FOXP3 were quantified with software‐based classifiers. Results: Reactive regions in early recurrent tumours contained more TAMs (31.4% vs 21.7%, P = 0.01), which showed increased expression of CD86 (59.4% vs 38.4%, P = 0.04), CD204 (48.5% vs 28.4%, P = 0.03), CD206 (25.5% vs 14.4%, P = 0.04) and increased staining intensity for CD163 (86.4 vs 57.7 arbitrary units, P = 0.02), compared to late recurring tumours. Reactive regions contained more B‐lymphocytes compared to patient‐matched primary tumours (0.71% vs 0.40%, P = 0.04). Fractions of total, cytotoxic and regulatory T‐lymphocytes did not differ. Conclusions: Early recurrent glioblastomas showed enrichment for TAMs, expressing both pro‐ and anti‐inflammatory markers and B‐lymphocytes. This may indicate a time‐dependent response to immunotherapy explained by time‐dependent alterations in the immune‐microenvironment in recurrent glioblastomas. [ABSTRACT FROM AUTHOR] |
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