Random and site-specific radiolabeling of [89Zr]Zr-DFO-anti-PD-L1-mAb iPET tracer.

Autor: Lin, Yi-Ching, Yang, Chao-Wei, Tsai, Shih-Chuan, Farn, Shiou-Shiow, Ou Yang, Fang-Yu, Lo, Wei-Lin, Chen, Liang-Cheng, Chen, Kuo-Ting, Weng, Mao-Chi, Kung, Jui-Yin, Qiu, Xin-Yu, Lu, Ching-Chun, Huang, Feng-Yun J.
Předmět:
Zdroj: Journal of Radioanalytical & Nuclear Chemistry; Nov2024, Vol. 333 Issue 11, p5997-6005, 9p
Abstrakt: Zirconium-89 radiolabeled immuno-positron emission tomography (89Zr-iPET) has become a critical tool for patient stratification before and after immunotherapies for therapeutic evaluation. In this study, random and site-specific radiolabeling of 89Zr-iPET tracers were investigated. Traditional lysine-based conjugation and enzyme-based GlyCLICK® kit method were used to prepare DFO-anti-PD-L1-mAb conjugates with a chelator-to-antibody ratio (CAR) of 0–7 and 2.0, respectively. Then, conjugates with different CAR levels were radiolabeled with 89Zr to obtain randomly or site-specifically radiolabeled 89Zr-iPET tracer. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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