| Abstrakt: |
The present study focuses on evaluating the probiotic potential and enzyme profiling of indigenous lactic acid bacteria. The cultures used were Lactiplantibacillus plantarum, two Lacticaseibacillus rhamnosus, Lactobacillus acidophilus, and Limosilactobacillus fermentum. In vitro analysis of acid and bile tolerance, cell surface hydrophobicity, aggregation potential, bile salt hydrolase activity, antimicrobial activities, and safety assessment were done for the isolates. All the cultures exhibited varying degrees of probiotic attributes confirming the strain specificity. The isolate Lactobacillus acidophilus, displayed the highest antimicrobial activity against both E. coli and S. aureus with zones of clearance measuring 16.50±0.50 mm and 19.00±1.00 mm, respectively. Enzyme profiling of the isolates was done for 19 enzymes and the results revealed the presence of four distinct enzymes in all cultures: leucine arylamidase, valine arylamidase, ß-galactosidase, and ß-glucosidase. [ABSTRACT FROM AUTHOR] |
