| Abstrakt: |
The purpose of this study was to investigate the distribution and assess the quantitative prevalence of various species of lactobacilli in the vaginal biocenosis of women in the Russian Federation with various physiological and dysbiotic conditions of the pelvic organs. During the research, 840 vaginal smears obtained from four cities of the Russian Federation were tested for the presence of lactobacilli and their species specificity. Smears were taken both for medical reasons and from apparently healthy women undergoing preventive checkups. Determination of the total content of bacteria and lactobacilli was carried out using quantitative polymerase chain reaction (PCR) with SYBR Green. Multiplex quantitative PCR using species-specific probes was used to detect and differentiate a total of 12 species of lactobacilli, potentially capable of colonizing the vaginal biocenosis. The most common species were L. iners (51.2%) and L. crispatus (43%), while L. jensenii (18.3%) and L. gasseri (7.9%) were seen less commonly. Other types of lactobacilli were sporadic. In 75% of cases, only one species of lactobacilli was predominant, whereas in 25% two to four species were co-dominating. Given a high proportion of lactobacilli in the total bacterial mass (>70%), L. crispatus was more common, while L. iners predominated if this proportion was low (<30%). However, no relationship was found between the age or region of residence of patients and the prevalence of Lactobacillus species. Only four species of lactobacilli, namely, L. crispatus, L. iners, L. jensenii, and L. gasseri, prevailed in the vaginal biocenosis of 97% of study participants, including both healthy women and women with various disorders of genital tract. [ABSTRACT FROM AUTHOR] |
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