| Autor: |
Kumar, Satendra, Nan, Liangliang, Kalodimou, Georgia, Jany, Sylvia, Freudenstein, Astrid, Brandmüller, Christine, Müller, Katharina, Girl, Philipp, Ehmann, Rosina, Guggemos, Wolfgang, Seilmaier, Michael, Wendtner, Clemens-Martin, Volz, Asisa, Sutter, Gerd, Fux, Robert, Tscherne, Alina |
| Předmět: |
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| Zdroj: |
International Journal of Molecular Sciences; Oct2024, Vol. 25 Issue 20, p10898, 20p |
| Abstrakt: |
The emergence of hitherto unknown viral pathogens presents a great challenge for researchers to develop effective therapeutics and vaccines within a short time to avoid an uncontrolled global spread, as seen during the coronavirus disease 2019 (COVID-19) pandemic. Therefore, rapid and simple methods to identify immunogenic antigens as potential therapeutical targets are urgently needed for a better pandemic preparedness. To address this problem, we chose the well-characterized Modified Vaccinia virus Ankara (MVA)-T7pol expression system to establish a workflow to identify immunogens when a new pathogen emerges, generate candidate vaccines, and test their immunogenicity in an animal model. By using this system, we detected severe acute respiratory syndrome (SARS) coronavirus 2 (SARS-CoV-2) nucleoprotein (N)-, and spike (S)-specific antibodies in COVID-19 patient sera, which is in line with the current literature and our observations from previous immunogenicity studies. Furthermore, we detected antibodies directed against the SARS-CoV-2-membrane (M) and -ORF3a proteins in COVID-19 patient sera and aimed to generate recombinant MVA candidate vaccines expressing either the M or ORF3a protein. When testing our candidate vaccines in a prime-boost immunization regimen in humanized HLA-A2.1-/HLA-DR1-transgenic H-2 class I-/class II-knockout mice, we were able to demonstrate M- and ORF3a-specific cellular and humoral immune responses. Hence, the established workflow using the MVA-T7pol expression system represents a rapid and efficient tool to identify potential immunogenic antigens and provides a basis for future development of candidate vaccines. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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