| Abstrakt: |
Morinda citrifolia L., commonly known as 'Noni', holds significant potential in pharmaceutical research due to its bioactive constituents. This study aimed to optimise the ultrasound-assisted extraction process for yield and total flavonoid content (TFC) from M. citrifolia fruits at different temperatures (40 °C, 50 °C, and 60 °C). The extraction kinetics were analysed using four models: firstorder kinetic, Peleg, power law, and two-site kinetic, determined through goodness-of-fit. Antioxidant activity was measured using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. Results showed the peak extraction yield at 40 °C within 90 min, yielding 1.46 ± 0.01 mg/mL, while the peak TFC was at 50 °C within 100 min, yielding 3.43 ± 0.00 mg CE/g db. The two-site kinetic model best described the extraction kinetics based on goodness of fit. The IC50 value for DPPH inhibition was 1883.57 ± 0.02 µg/mL at 50 °C, indicating moderate antioxidant activity compared to ascorbic acid (95.11 ± 0.13 µg/mL). Overall, M. citrifolia fruits provide valuable insights for standardised extraction methods, potentially benefiting therapeutic applications and indirectly reducing operational costs and time for manufacturers. [ABSTRACT FROM AUTHOR] |
